The invention relates to the use of G-CSF in combination with a chemotherapeutic agent (in particular, cyclophosphamide) to produce a pharmaceutical preparation for boosting the mobilization of hematopoietic stem cells from bone marrow in the treatment of diseases requiring peripheral stem cell transplantation. The claimed combination results in more efficient leukapheresis, e.g. before myeloblative or myelotoxic therapy.
* Claims
What is claimed is:
1. A method of treating a disease requiring peripheral stem cell transplantation in a patient in need of such treatment, comprising administering to the patient a hematopoietic stem cell mobilizing-effective amount of G-CSF; and thereafter administering to the patient a disease treating-effective amount of at least one chemotherapeutic agent.
2. The method of claim 1, wherein the disease is a tumor disease.
3. The method of claim 1, wherein the G-CSF is recombinant G-CSF.
4. The method of claim 1, wherein the at least one chemotherapeutic agent opens the endothelial barrier of the patient to render the endothelial barrier permeable for stem cells.
5. The method of claim 1, wherein the at least one chemotherapeutic agent is cyclophosphamide.
6. The method of claim 1, wherein the G-CSF is administered once per day over 2-3 consecutive days, and the chemotherapeutic agent is administered immediately after the final administration of G-CSF, or on a fourth consecutive day.
7. A pharmaceutical kit, comprising
a first component comprising G-CSF;
a second component comprising at least one chemotherapeutic agent; and
a third component comprising instructions for the administration of the G-CSF prior to the onset of administration of the at least one chemotherapeutic agent.
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